Journal
OPTICS LETTERS
Volume 39, Issue 8, Pages 2431-2434Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.39.002431
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Funding
- Biotechnology and Biological Sciences Research Council UK [BB/I022074/1, BB/I022937/1]
- STMicroelectronics (Imaging Division, Edinburgh, UK)
- Medical Research Council UK [MR/K015664/1]
- Dimbleby Cancer Care endowment fund
- European Community
- Biotechnology and Biological Sciences Research Council [BB/I022937/1, BB/I022074/1] Funding Source: researchfish
- Medical Research Council [MR/K015664/1] Funding Source: researchfish
- BBSRC [BB/I022074/1, BB/I022937/1] Funding Source: UKRI
- MRC [MR/K015664/1] Funding Source: UKRI
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We report on the development of a doubly weighted Gerchberg-Saxton algorithm (DWGS) to enable generation of uniform beamlet arrays with a spatial light modulator (SLM) for use in multiphoton multifocal imaging applications. The algorithm incorporates the WGS algorithm as well as feedback of fluorescence signals from the sample measured with a single-photon avalanche diode (SPAD) detector array. This technique compensates for issues associated with nonuniform illumination onto the SLM, the effects due to aberrations and the variability in gain between detectors within the SPAD array to generate a uniformly illuminated multiphoton fluorescence image. We demonstrate the use of the DWGS with a number of beamlet array patterns to image muscle fibers of a 5-day-old fixed zebrafish larvae. (C) 2014 Optical Society of America
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