Journal
OPTICS LETTERS
Volume 36, Issue 17, Pages 3389-3391Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.36.003389
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Funding
- National Science Foundation (NSF) [0852742]
- W. M. Keck Center for Adaptive Optical Microscopy at UC Santa Cruz
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0852742] Funding Source: National Science Foundation
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We introduce a direct wavefront sensing method using structures labeled with fluorescent proteins in tissues as guide stars. An adaptive optics confocal microscope using this method is demonstrated for imaging of mouse brain tissue. A dendrite and a cell body of a neuron labeled with yellow fluorescent protein are tested as guide stars without injection of other fluorescent labels. Photobleaching effects are also analyzed. The results shows increased image contrast and 3x improvement in the signal intensity for fixed mouse tissues at depths of 70 mu m. (C) 2011 Optical Society of America
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