Journal
OPTICS LETTERS
Volume 36, Issue 2, Pages 202-204Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.36.000202
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Funding
- National Institute of General Medical Sciences [R01GM085437]
- National Science Foundation (NSF)
- 3Com Corporation
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We describe the corkscrew point spread function (PSF), which can localize objects in three dimensions throughout a 3.2 mu m depth of field with nanometer precision. The corkscrew PSF rotates as a function of the axial (z) position of an emitter. Fisher information calculations show that the corkscrew PSF can achieve nanometer localization precision with limited numbers of photons. We demonstrate three-dimensional super-resolution microscopy with the corkscrew PSF by imaging beads on the surface of a triangular polydimethylsiloxane (PDMS) grating. With 99,000 photons detected, the corkscrew PSF achieves a localization precision of 2.7 nm in x, 2.1 nm in y, and 5.7 nm in z. (C) 2011 Optical Society of America
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