Journal
OPTICS LETTERS
Volume 36, Issue 12, Pages 2236-2238Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.36.002236
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Droplet-based microfluidic systems enable miniaturization of chemical reactions in femtoliter to picoliter volume compartments. Quantifying mixing dynamics of the reagents in droplets is critical to determine the system performance. In this Letter, we developed a two-photon excitation fluorescence lifetime imaging technique to quantitatively image the mixing dynamics in microfluidic droplets. A cross/autocorrelation method was used to reconstruct a high-quality fluorescence lifetime image of the droplet. The fluorescence decay was analyzed for accurate determination of the mixing ratio at each pixel of the image. (C) 2011 Optical Society of America
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