Journal
OPTICS LETTERS
Volume 35, Issue 19, Pages 3195-3197Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.35.003195
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Funding
- National Institutes of Health (NIH) [R01 EB000712, R01 EB008085, R01 CA113453901, U54 CA136398, 5P60 DK02057933]
- Microphotoacoustics, Inc.
- Endra, Inc.
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Optical absorption provides essential biological functional information but cannot be sensed by mainstream optical microscopy technologies directly, which detect fluorescence or scattering and may require undesirable labeling. Here we developed in vivo subwavelength-resolution photoacoustic microscopy (SW-PAM) that provides exquisitely high optical-absorption contrast due to nonfluorescent, or fluorescent, endogenous pigments. Having approached the ultimate diffraction-limited optical resolution, SW-PAM can resolve subcellular organelles. Vasculature and early-stage melanoma were imaged with 12:1 and 17:1 contrasts, respectively, without labeling. SW-PAM along with the scaled-up macroscopy, as the only technology that measures the same contrast origin over such a wide length scale, can potentially accelerate translation from microscopic research to clinical practice. (C) 2010 Optical Society of America
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