Journal
OPTICS LETTERS
Volume 35, Issue 24, Pages 4139-4141Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.35.004139
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Funding
- National Institutes of Health (NIH) [R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398]
- Microphotoacoustics, Inc.
- Endra, Inc.
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Imaging of cell nuclei plays a critical role in cancer diagnosis and prognosis. To image noninvasively cell nuclei in vivo without staining, we developed UV photoacoustic microscopy (UV-PAM), in which 266 nm wavelength UV light excites unlabeled DNA and RNA in cell nuclei to produce photoacoustic waves. We applied UV-PAM to ex vivo imaging of cell nuclei in a mouse lip and a mouse small intestine and to in vivo imaging of the cell nuclei in the mouse skin. The UV-PAM images of unstained cell nuclei match the optical micrographs of the histologically stained cell nuclei. Given intrinsic optical contrast and high spatial resolution, in vivo label-free UV-PAM has potential for unique biological and clinical application. (C) 2010 Optical Society of America
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