Journal
OPTICS LETTERS
Volume 33, Issue 16, Pages 1819-1821Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.33.001819
Keywords
-
Categories
Funding
- National Institutes of Health NIH [R21 EB007338]
Ask authors/readers for more resources
We describe a method of obtaining optical sectioning with a standard wide-field fluorescence microscope. The method involves acquiring two images, one with nonuniform illumination (in our case, speckle) and another with uniform illumination (in our case, randomized speckle). An evaluation of the local contrast in the speckle-illumination image provides an optically sectioned image with low resolution. This is complemented with high-resolution information obtained from the uniform-illumination image. A fusion of both images leads to a full resolution image that is optically sectioned across all spatial frequencies. This hybrid illumination method is fast, robust, and generalizable to a variety of illumination and imaging configurations. (c) 2008 Optical Society of America.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available