Journal
OPTICS EXPRESS
Volume 22, Issue 6, Pages 7028-7039Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.22.007028
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- European Union through the ERC starting grant Remodeling
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We describe the design and implementation of a stimulated emission depletion (STED) microscope which allows simultaneous three-dimensional super-resolution imaging in two colors. A super-continuum laser source is used to provide all spectral bands necessary for excitation and efficient depletion to achieve a lateral and axial resolution of similar to 35 nm and similar to 90 nm respectively. We characterize the systems' performance by imaging colloidal particles and single fluorescent molecules. Its biological applicability is demonstrated by dual-color imaging of nuclear pore complexes and of DNA replication sites in mammalian cells. (C)2014 Optical Society of America
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