Journal
OPTICS EXPRESS
Volume 22, Issue 19, Pages 22561-22574Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.22.022561
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Funding
- INCa (French National Institute of cancer) [PAIR GYNECO 2012-1-GYN-01-EP]
- French National Agency for Research (ANR) [ANR-10-INSB-04]
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We studied the azimuthal orientations of collagen fibers in histological slides of uterine cervical tissue by two different microscopy techniques, namely Mueller polarimetry (MP) and Second Harmonic Generation (SHG). SHG provides direct visualization of the fibers with high specificity, which orientations is then obtained by suitable image processing. MP provides images of retardation (among other polarimetric parameters) due to the optical anisotropy of the fibers, which is enhanced by Picrosirius Red staining. The fiber orientations are then assumed to be those of the retardation slow axes. The two methods, though fully different from each other, provide quite similar maps of average fiber orientations. Overall, our results confirm that MP microscopy provides reliable images of dominant fiber orientations at a much lower cost that SHG, which remains the gold standard for specific imaging of collagen fibers using optical microscopy. (C) 2014 Optical Society of America
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