Journal
OPTICS EXPRESS
Volume 21, Issue 13, Pages 16007-16021Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.21.016007
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Funding
- Kavli Institute for Brain Science
- NEI
- NINDS
- NIHM
- NIDA
- Keck Foundation
- NARSAD
- U. S. Army Research Laboratory
- U. S. Army Research Office [W911NF-12-1-0594]
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Imaging three-dimensional structures represents a major challenge for conventional microscopies. Here we describe a Spatial Light Modulator (SLM) microscope that can simultaneously address and image multiple targets in three dimensions. A wavefront coding element and computational image processing enables extended depth-of-field imaging. High-resolution, multi-site three-dimensional targeting and sensing is demonstrated in both transparent and scattering media over a depth range of 300-1,000 microns. (C) 2013 Optical Society of America
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