Journal
OPTICS EXPRESS
Volume 20, Issue 9, Pages -Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.20.009673
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Funding
- KAIST, KAIST Institute for Optical Science and Technology, the Korean Ministry of Education, Science and Technology (MEST) [2009-0087691]
- TJ ChungAm Foundation
- Ministry of Education, Science & Technology (MoST), Republic of Korea [KAIST_HR_2012_18, G04100075] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Ministry of Science, ICT & Future Planning, Republic of Korea [KIOST01] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2009-0087691] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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We report a technique for simultaneous label-free quantification of cytoplasmic hemoglobin Hb concentration and dynamic membrane fluctuation in individual red blood cells (RBCs). Spectroscopic phase microscopy equipped with three different coherent laser sources and a color detector records three wavelength-dependent quantitative phase images in a single shot of a color-coded hologram. Using molecular specific dispersion, we demonstrate the extraction of Hb concentration and the dynamic membrane fluctuation from individual RBCs. (c) 2012 Optical Society of America
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