4.6 Article

Adaptive optics enables 3D STED microscopy in aberrating specimens

Journal

OPTICS EXPRESS
Volume 20, Issue 19, Pages 20998-21009

Publisher

OPTICAL SOC AMER
DOI: 10.1364/OE.20.020998

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Funding

  1. Wellcome Trust [095927/A/11/Z, 095927/B/11/Z]
  2. Kavli Foundation
  3. National Institute of General Medical Sciences [F32GM096859]
  4. Engineering and Physical Sciences Research Council, UK [EP/E055818/1]
  5. EPSRC [EP/E055818/1] Funding Source: UKRI
  6. Wellcome Trust [095927/B/11/Z] Funding Source: Wellcome Trust
  7. Engineering and Physical Sciences Research Council [EP/E055818/1] Funding Source: researchfish

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Stimulated emission depletion (STED) microscopy allows fluorescence far-field imaging with diffraction-unlimited resolution. Unfortunately, extending this technique to three-dimensional (3D) imaging of thick specimens has been inhibited by sample-induced aberrations. Here we present the first implementation of adaptive optics in STED microscopy to allow 3D super-resolution imaging in strongly aberrated imaging conditions, such as those introduced by thick biological tissue. (C) 2012 Optical Society of America

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