Journal
OPTICS EXPRESS
Volume 20, Issue 3, Pages 3057-3065Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.20.003057
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Funding
- National Institutes of Health [5T90 DA022871]
- Photosynthetic Antenna Research Center (PARC), an Energy Frontier Research Center
- US Department of Energy [DE-SC 0001035]
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Bio-mechanism investigations demand single particle tracking with high spatial and temporal resolutions which require single fluorophore 3D localization measurements with matching precision and speed. Although the precision for lateral-localization measurements is well described by an analytical expression, for the axial direction, it is often obtained by repeating location measurements or by estimating a lower bound. Here, we report a precision expression for an axial-localization method that analyzes the standard deviations of single fluorophores' intensity profiles. Like the lateral-localization precision, this expression includes all relevant experimental effects measurable from a Gaussian intensity profile of the fluorophore. This expression completes the precision analysis for single-image 3D localization of individual fluorophores and lifts the temporal resolution to the typical exposure timescales of milliseconds. (C) 2012 Optical Society of America
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