Journal
OPTICS EXPRESS
Volume 19, Issue 4, Pages 3130-3143Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.19.003130
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- European Community [201837]
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We describe a STED microscope optimized for colocalization experiments with up to three colors. Two fluorescence labels are separated by their fluorescence lifetime whereas a third channel is discriminated by the wavelength of fluorescence emission. Since it does not require a second STED beam, separating by lifetime is insensitive to drift and thus optimally suited for colocalization analyses. Furthermore, we propose a setup having a second STED beam for long duration multicolor recording. (C) 2011 Optical Society of America
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