Journal
OPTICS EXPRESS
Volume 19, Issue 18, Pages 17143-17150Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.19.017143
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Funding
- NSERC [355544-2008, 375340-2009, STPGP 396444]
- Terry-Fox Foundation
- Canadian Cancer Society [TFF 019237, TFF 019240, CCS 2011-700718]
- Alberta Cancer Research Institute [ACB 23728]
- Canada Foundation for Innovation
- Leaders Opportunity Fund [18472]
- Alberta Advanced Education Technology
- Small Equipment Grants Program [URSI09007SEG]
- Microsystems Technology Research Initiative [MSTRI RES0003166]
- University of Alberta
- Alberta Ingenuity / Alberta Innovates scholarships
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Optical-resolution photoacoustic microscopy (OR-PAM) is capable of achieving optical-absorption-contrast images with micron-scale spatial resolution. Previous OR-PAM systems have been frame-rate limited by mechanical scanning speeds and laser pulse repetition rate (PRR). We demonstrate OR-PAM imaging using a diode-pumped nanosecond-pulsed Ytterbium-doped 532-nm fiber laser with PRR up to 600 kHz. Combined with fast-scanning mirrors, our proposed system provides C-scan and 3D images with acquisition frame rate of 4 frames per second (fps) or higher, two orders of magnitude faster than previously published systems. Highcontrast images of capillary-scale microvasculature in a live Swiss Webster mouse ear with similar to 6-mu m optical lateral spatial resolution are demonstrated. (C) 2011 Optical Society of America
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