Journal
OPTICS EXPRESS
Volume 17, Issue 8, Pages 5987-5997Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.17.005987
Keywords
-
Categories
Funding
- National Research Program for Genomic Medicine (NRPGM)
- National Science Council (NSC) in Taiwan [97-3112-B-006-013]
Ask authors/readers for more resources
A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide fluorescent images of living cell membranes. The proposed microscope with the help of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having a shorter fluorophore lifetime. In comparison with a one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. By combining the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated it's capability for brighter and more contrasted fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct. (C) 2009 Optical Society of America
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
