Journal
OPTICS EXPRESS
Volume 16, Issue 21, Pages 16984-16992Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.16.016984
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Funding
- OSDA
- Free State of Thuringia (Germany)
- European Regional Development Fund
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The optical cell rotator (OCR) is a modified dual-beam laser trap for the holding and controlled rotation of suspended dielectric microparticles, such as cells. In contrast to optical tweezers, OCR uses two counter-propagating divergent laser beams, which are shaped and delivered by optical fibers. The rotation of a trapped specimen is carried out by the rotation of a dual-mode fiber, emitting an asymmetric laser beam. Experiments were performed on human erythrocytes, promyelocytic leukemia cells (HL60), and cell clusters (MCF-7). Since OCR permits the rotation of cells around an axis perpendicular to the optical axis of any microscope and is fully decoupled from imaging optics, it could be a suitable and expedient tool for tomographic microscopy. (C) 2008 Optical Society of America
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