Journal
OPTICS EXPRESS
Volume 16, Issue 26, Pages 21843-21848Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.16.021843
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Funding
- Biotechnology and Biological Sciences Research Council (BBSRC)
- Biotechnology and Biological Sciences Research Council [BB/F016042/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/E055818/1] Funding Source: researchfish
- BBSRC [BB/F016042/1] Funding Source: UKRI
- EPSRC [EP/E055818/1] Funding Source: UKRI
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We describe an optical microscope system whose focal setting can be changed quickly without moving the objective lens or specimen. Using this system, diffraction limited images can be acquired from a wide range of focal settings without introducing optical aberrations that degrade image quality. We combine this system with a real time Nipkow disc based confocal microscope so as to permit the acquisition of extended depth of field images directly in a single frame of the CCD camera. We also demonstrate a simple modification that enables extended depth of field images to be acquired from different angles of perspective, where the angle can be changed over a continuous range by the user in real-time. (C) 2008 Optical Society of America
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