Journal
OPTICS EXPRESS
Volume 16, Issue 25, Pages 20848-20863Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.16.020848
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Funding
- National Institutes of Health [R01-EY11289-23, R01-NS057476-02, R01-CA75289-12, R01NS057476- 01A1, P01-NS05S104-01A1, 4-R00-AI073457]
- National Science Foundation [BES-0522845]
- Air Force Office of Scientific Research [FA9550-07-1-0101]
- Medical Free Electron Laser Program [FA9550-07-1-0101]
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Multi-photon microscopy (MPM) is a powerful tool for biomedical imaging, enabling molecular contrast and integrated structural and functional imaging on the cellular and subcellular level. However, the cost and complexity of femtosecond laser sources that are required in MPM are significant hurdles to widespread adoption of this important imaging modality. In this work, we describe femtosecond diode pumped Cr:LiCAF laser technology as a low cost alternative to femtosecond Ti:Sapphire lasers for MPM. Using single mode pump diodes which cost only $150 each, a diode pumped Cr:LiCAF laser generates similar to 70-fs duration, 1.8-nJ pulses at similar to 800 nm wavelengths, with a repetition rate of 100 MHz and average output power of 180 mW. Representative examples of MPM imaging in neuroscience, immunology, endocrinology and cancer research using Cr:LiCAF laser technology are presented. These studies demonstrate the potential of this laser source for use in a broad range of MPM applications. (C) 2008 Optical Society of America
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