Journal
OPTICS EXPRESS
Volume 16, Issue 17, Pages 12640-12649Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.16.012640
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Funding
- National Cancer Institute
- National Institutes of Health [1 R33 CA112141, 1 R21 RR021893]
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We report on the utilization of a dual-clad optical fiber for two-photon excited fluorescence correlation spectroscopy (FCS). High excitation efficiency is obtained by sending prechirped ultrafast pulses through the single-mode core of the fiber, while the fluorescence collection efficiency is enhanced because of the larger numerical aperture of the inner cladding. We show that the utilization of a dual-clad fiber is critical for ultrasensitive two-photon fluorescence detection. Our system has the ability to detect fluorescent nanospheres as small as 12 nm in radius. Quantum dots of radius 7 nm are also measured and show excellent signal to noise ratio. The particle sizes obtained from the fiber FCS system were confirmed by measurements using a commercial dynamic light scattering (DLS) system. (c) 2008 Optical Society of America.
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