Journal
OPTICS COMMUNICATIONS
Volume 285, Issue 6, Pages 1404-1407Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.optcom.2011.10.028
Keywords
Forster-Resonant-Energy-Transfer (FRET); Superresolution; Microscopy
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Funding
- NSFC [61078016]
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Saturated Forster-Resonant-Energy-Transfer (FRET) microscopy (SFM) employing tiny silica nanoparticles densely co-doped with Cy3 and Cy5 molecules as fluorescent labels has been proposed and analyzed with rigorous rate equations previously, showing that optical resolutions beyond the diffraction-limit in both lateral and axial directions can be realized by exploiting the saturation effect in FRET process. Here, using a set of similar rate equations, we show that SFM can also be realized with single FRET dye pairs as fluorescent labels. A similar nonlinear optical response to excitation beam is revealed for Cy3-Cy5 dye pairs, in comparison to the silica nanoparticles co-doped with Cy3 and Cy5 molecules. (C) 2011 Elsevier B.V. All rights reserved.
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