Journal
OPTICS COMMUNICATIONS
Volume 281, Issue 4, Pages 880-887Publisher
ELSEVIER
DOI: 10.1016/j.optcom.2007.10.007
Keywords
sectioning microscopy; high numerical aperture theory
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Funding
- BBSRC [BB/F016042/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F016042/1] Funding Source: researchfish
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We describe the theory of a new method of optical refocusing that is particularly relevant for confocal and multiphoton microscopy systems. This method avoids the spherical aberration that is common to other optical refocusing systems. We show that aberration-free refocusing can be achieved over an axial scan range of 70 mu m for a 1.4 NA objective lens. As refocusing is implemented remotely from the specimen, this method enables high axial scan speeds without mechanical interference between the objective lens and the specimen. (C) 2007 Elsevier B.V. All rights reserved.
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