Journal
OPTICS AND LASERS IN ENGINEERING
Volume 50, Issue 10, Pages 1431-1435Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.optlaseng.2012.05.002
Keywords
Digital microscopic holography; 3D profile reconstruction; Phase-shifting technique; Image correlation algorithm; Phase aberration compensation
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Funding
- National Nature Science Foundation of China [61078064, 60978065, 60877070]
- National University Student Innovation Program [52400415]
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To improve the measuring accuracy is an important research content for digital microscopic holography (DMH) development and application. In this study, we have upgraded application of DMH through the in-line image-plane phase-shifting technique and the image correlation algorithm to reconstruct the 3D profile of a biological sample. Importantly, since this novel DMH system can obtain the phase-shifting hologram with a high ratio of signal to noise conveniently, the reconstructed algorithm of DMH and the compensation operation of the phase aberration are simplified significantly. Moreover, by using the image correlation algorithm, the digital phase mask with high precision also can be obtained easily; thus both the measuring accuracy of DMH and the quality of the reconstructed image are improved significantly. More importantly, this kind of in-line image-plane phase-shifting digital microscopic holography provides a powerful imaging tool to simultaneously reconstruct the amplitude and the phase of the measured object with submicron scale resolution. (C) 2012 Elsevier Ltd. All rights reserved.
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