Journal
OPHTHALMIC RESEARCH
Volume 40, Issue 3-4, Pages 124-128Publisher
KARGER
DOI: 10.1159/000119862
Keywords
age-related macular degeneration; animal model; CCL2; CX3CR1; retinal pigment epithelium; N-retinylidene-N-retinylethanolamine; chaperone; omega-3 long-chain polyunsaturated fatty acids
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Funding
- Intramural NIH HHS [Z01 EY000418-04] Funding Source: Medline
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Background/Aims: Senescent Ccl2(-/-) mice develop cardinal features of human age-related macular degeneration (AMD). Loss-of-function single-nucleotide polymorphisms within CX3CR1 are associated with AMD. Methods: We generated Ccl2(-/-)/Cx3cr1(-/-) [double-knockout (DKO)] mice and evaluated the eyes using fundoscopy routine histology, immunochemistry, biochemistry and proteomics. Results: At 6 weeks old, all DKO mice developed AMD-like retinal lesions such as abnormal retinal pigment epithelium cells, drusen, photoreceptor atrophy and choroidal neovascularization, which progressed with age and reversed with high omega-3 long-chain polyunsaturated fatty acid diet. N-retinylidene-N-retinylethanolamine (A2E), a major lipofuscin fluorophore, illustrated by an emission peak at similar to 600 nm, was significantly higher in DKO retinal pigment epithelium. Decreased ERp29 was found in the retina of DKO mice. Conclusion: A broad spectrum of AMD pathologies with early onset and high penetrance in these mice implicate certain chemokines, A2E and endoplasmic reticulum proteins in AMD pathogenesis. Copyright (c) 2008 S. Karger AG, Basel.
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