Journal
ONCOLOGY REPORTS
Volume 30, Issue 6, Pages 2999-3005Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2013.2747
Keywords
valproic acid; histone deacetylase; apoptosis; HeLa; caspase
Categories
Funding
- National Research Foundation of Korea (NRF)
- Korean government through the Diabetes Research Center at Chonbuk National University [2012-0009323]
- Basic Science Research Program through the National Research Foundation of Korea (NRF)
- Ministry of Education [2013006279]
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Valproic acid (VPA) as a histone deacetylase (HDAC) inhibitor has an anticancer effect. In the present study, we evaluated the effects of VPA on the growth and death of HeLa cervical cancer cells in relation to reactive oxygen species (ROS) and glutathione (GSH). Dose- and time-dependent growth inhibition was observed in HeLa cells with an IC50 of approximately 10 mM at 24 h. DNA flow cytometric analysis indicated that 10 mM VPA induced a G2/M phase arrest of the cell cycle. This agent also induced apoptosis, which was accompanied by the cleavage of PARP, the activation of caspase-3, -8 and -9, and the loss of mitochondrial membrane potential (MMP; (m)). All the tested caspase inhibitors significantly prevented HeLa apoptotic cell death induced by VPA, whereas TNF- intensified the apoptotic cell death. With respect to ROS and GSH levels, VPA increased ROS levels and induced GSH depletion. However, N-acetyl cysteine (NAC; an antioxidant) and L-buthionine sulfoximine (BSO; a GSH synthesis inhibitor) did not significantly affect cell death in VPA-treated HeLa cells. In conclusion, VPA inhibits the growth of HeLa cervical cancer cells via caspase-dependent apoptosis and the growth inhibition is independent of ROS and GSH level changes.
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