Journal
ONCOLOGY REPORTS
Volume 29, Issue 1, Pages 169-176Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2012.2105
Keywords
esophageal carcinoma; microRNA; microarray; profile
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Funding
- National Natural Science Foundation of China [81172747, 81072259, 30800891, 81111140396]
- Research Fund for the Doctoral Program of Higher Education of China [200802861045]
- Natural Science Foundation of Jiangsu province, China [BK2010407]
- Research and Teaching Fund for the Excellent Youth Scholars of Southeast University
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Esophageal squamous cell carcinoma (ESCC) is one of the most lethal malignancies worldwide. To reduce the high morbidity and mortality of the disease, sensitive and specific biomarkers for early detection are urgently needed. Tumor-specific microRNAs (miRNAs) seem to be potential biomarkers for the early diagnosis and treatment of cancer. In this study, differentially expressed miRNAs in tumor tissues and adjacent non-tumor tissues were detected by miRNA microarrays. Stem-loop real-time reverse transcription PCR was conducted to verify the candidate miRNAs discovered by microarray analysis. The data showed that hsa-miR-338-3p, hsa-miR-218 and hsa-miR-139-5p were downregulated in tumor tissues compared with adjacent non-tumor tissues, while hsa-miR-183, hsa-miR-574-5p, hsa-miR-21* and hsa-miR-601 were upregulated in tumor tissues. Multiple regression analysis revealed the aberrant expression of hsa-miR-338-3p, hsa-miR-139-5p, hsa-miR-574-5p and hsa-miR-601 increased the risk of esophageal cancer. Furthermore, we found hsa-miR-21* was significantly increased in heavy drinking patients. Therefore, there is a set of differentially expressed miRNAs in esophageal cancer which may be associated with the incidence and development of ESCC. Differential expression profiles of miRNAs in ESCC may be promising biomarkers for the early screening of high-risk populations and early detection.
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