Characterization of the low pH/low nutrient-resistant LNCaP cell subline LNCaP-F10

Intratumoral regions of low extracellular pH and low nutrition are common features of solid tumors. Although cancer cells normally die when they are removed from their environment, a small population of cells survive. In the present study, the subline LNCaP-F10, of the prostate cancer cell line LNCaP, was isolated and its low pH/low nutrient-resistant properties were examined. LNCaP-F10 cells were grown under low-pH/low-nutrient conditions, which caused cell death of the LNCaP cells. The cell death was associated with oligonucleosomal DNA fragmentation and poly (ADP-ribose) polymerase (PARP) cleavage, indicating that low-pH/low-nutrient induced apoptosis in these cells. Significant differences in the expression of BCL2, BIRC5 and DARK1 were detected between LNCaP-F10 and LNCaP cells. Tumor growth caused by implantation of LNICaP-F10 cells into the renal subcapsular space of nude mice in the absence or presence of prostate stromal cell stimulation was greater than that caused by implantation of LNCaP cells. LNCaP-F10 cells were resistant to apoptosis induced by an environment of low-pH/low-nutrient in vitro, and displayed malignant potential in vivo.