Journal
ONCOLOGY REPORTS
Volume 29, Issue 1, Pages 253-259Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2012.2083
Keywords
epigallocatechin gallate; dermal fibroblast; UVB irradiation; miRNA expression; cell viability
Categories
Funding
- Ministry of Education, Science and Technology of Republic of Korea [20110028646]
Ask authors/readers for more resources
The green tea polyphenol epigallocatechin-3-gallate (EGCG) is a potent anti-oxidant and anticancer compound. Recently, EGCG-mediated UVB photoprotection was reported in normal human dermal fibroblasts (NHDFs); however, the molecular mechanism underlying this process remains unknown. Thus, we investigated the EGCG-specific microRNAs (miRNAs) involved in the UVB protective response in NHDFs. WST-1 assays confirmed that low doses (<50 mu M) of EGCG were non-cytotoxic and capable of recovering NHDF cell viability following UVB irradiation up to 83.7% compared to the control cells. Microarray analysis identified several miRNAs that were upregulated and downregulated significantly in this UVB protective response, with downregulated miRNAs outnumbering the upregulated ones. Bioinformatic studies, including miRNA target gene prediction and gene ontology analysis, revealed that the EGCG-specific miRNAs may control genes involved in transcription regulation and inhibition of apoptosis, but not MAPK activation, in NHDFs. Therefore, these results suggest that EGCG may serve as a potential natural photoprotective agent against UVB-mediated damage in NHDFs by altering specific miRNA expression.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available