Journal
ONCOLOGY REPORTS
Volume 29, Issue 1, Pages 349-354Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2012.2093
Keywords
D-limonene; Akt; apoptosis
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Funding
- Natural Science Foundation of Heilongjiang Province [D2007-79]
- Natural Science Foundation for Young Scientists of Heilongjiang Province [QC2008C23]
- Science Research Foundation of the Health Department of Heilongjiang [2006-175]
- Heilongjiang Postdoctoral Science-Research Foundation
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D-limonene is recognized as a potential chemotherapeutic agent, however, the details of this mechanism remain unclear. In this study, we investigated the effects of D-limonene on colon cancer cell viability and its potential mechanism of action in vitro. After 48 h of treatment, D-limonene suppressed the viability of LS174T cells in a dose-dependent manner and caused a dose-dependent apoptotic cell death. D-limonene activated caspase-3 and -9 and PARP cleavage in a dose-dependent manner. Moreover, an increase in Bax protein and cytosol cytochrome c from mitochondria and a decrease in Bcl-2 protein were observed following treatment with D-limonene. In addition, D-limonene decreased the levels of p-Akt (Ser473), p-Akt (Thr308) and p-GSK-3 beta (Ser9), suggesting that D-limonene induced apoptosis via the mitochondrial death pathway and the suppression of the PI3K/Akt pathway.
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