Journal
ONCOGENE
Volume 31, Issue 28, Pages 3346-3356Publisher
SPRINGERNATURE
DOI: 10.1038/onc.2011.505
Keywords
MUC4; pancreatic cancer; metastasis; NIDO domain; MUC4 domains
Funding
- National Institutes of Health [CA78590, CA111294, CA127297, CA133774, CA131944]
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MUC4 is a large transmembrane type I glycoprotein that is overexpressed in pancreatic cancer (P =C) and has been shown to be associated with its progression and metastasis. However, the exact cellular and molecular mechanism(s) through which MUC4 promotes metastasis of PC cells has been sparsely studied. Here we showed that the nidogen-like (NIDO) domain of MUC4, which is similar to the G1-domain present in the nidogen or entactin (an extracellular matrix protein), contributes to the protein-protein interaction property of MUC4. By this interaction, MUC4 promotes breaching of basement membrane (BM) integrity, and spreading of cancer cells. These observations are corroborated with the data from our study using an engineered MUC4 protein without the NIDO domain, which was ectopically expressed in the MiaPaCa PC cells, lacking endogenous MUC4 and nidogen protein. The in vitro studies demonstrated an enhanced invasiveness of MiaPaCa cells expressing MUC4 (MiaPaCa-MUC4) compared with vector-transfected cells (MiaPaCa-Vec; P = 0.003) or cells expressing MUC4 without the NIDO domain (MiaPaCa-MUC4-NIDOD; P = 0.03). However, the absence of NIDO-domain has no significant role on cell growth and motility (P = 0.93). In the in vivo studies, all the mice orthotopically implanted with MiPaCa-MUC4 cells developed metastasis to the liver as compared with MiaPaCa-Vec or the MiaPaCaMUC4- NIDOD group, hence, supporting our in vitro observations. Additionally, a reduced binding (P = 0.0004) of MiaPaCa-MUC4-NIDOD cells to the fibulin-2 coated plates compared with MiaPaCa-MUC4 cells indicated a possible interaction between the MUC4-NIDO domain and fibulin-2, a nidogen-interacting protein. Furthermore, in PC tissue samples, MUC4 colocalized with the fibulin-2 present in the BM. Altogether, our findings demonstrate that the MUC4-NIDO domain significantly contributes to the MUC4-mediated metastasis of PC cells. This may be partly due to the interaction between the MUC4-NIDO domain and fibulin-2. Oncogene (2012) 31, 3346-3356; doi: 10.1038/onc.2011.505; published online 21 November 2011
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