Journal
OECOLOGIA
Volume 173, Issue 4, Pages 1439-1450Publisher
SPRINGER
DOI: 10.1007/s00442-013-2734-4
Keywords
Ectomycorrhizal pine roots; Carbon-nitrogen exchange; Carbon-13 stable isotope; Nitrogen-15 stable isotope; Nitrogen fertilization
Categories
Funding
- NSF Biocomplexity grant [DEB-9981711]
- Kearney Foundation student fellowship
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Ectomycorrhizal (EM) fungi form relationships with higher plants; plants transfer C to fungi, and fungi transfer nutrients to their host. While evidence indicates that this interaction is largely mutualistic, less is known about how nutrient supply and EM associates may alter C and nutrient exchanges, especially in intact plant-soil-microbe systems in the field. In a dual-labeling experiment with N fertilization, we used C and N stable isotopes to examine in situ transfers in EM pine trees in a Pinus sabiniana woodland in northern California. We added (NH4SO2)-N-15 and (CO2)-C-13 to track C-13 transfer from pine needles to EM roots and N-15 transfer from soil to EM roots and pine needles. Transfers of C-13 and N-15 differed with EM morphotype and with N fertilization. The brown morphotype received the least C per unit of N transferred (5:1); in contrast red and gold morphotypes gained more C and transferred less N (17:1 and 25:1, respectively). N fertilization increased N retention by ectomycorrhizas (EMs) but did not increase N transfer from EMs to pine needles. Therefore N fertilization positively affected both nutrient and C gains by EMs, increasing net C flows and N retention in EMs. Our work on intact and native trees/EM associations thereby extends earlier conclusions based on pot studies with young plants and culturable EM fungi; our results support the concept that EM-host relationships depend on species-level differences as well as responses to soil resources such as N.
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