Journal
TECHNOLOGY IN CANCER RESEARCH & TREATMENT
Volume 14, Issue 4, Pages 383-394Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/1533034614500416
Keywords
Cancer classification; CIMP; DNA methylation; Promoter; Targeted bisulfite sequencing
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Funding
- State Key Development Program for Basic Research of China-973 Program [2011CB809201]
- National High Technology Research and Development Program of China-863 Program [2012AA02A201, 2012AA02A502]
- National Natural Science Foundation of China [31161130357]
- Guangdong Enterprise Key Laboratory of Human Disease Genomics
- Guangdong Innovative Research Team Program [2009010016]
- China National GeneBank-Shenzhen
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DNA methylation plays a significant role in assuring cell identity, thus potentiating its application in molecular classification of cancers in respect to tissue-origins or clinically and etiologically distinct subtypes. In this study, we optimized our liquid hybridization capture-based bisulfite sequencing (LHC-BS) approach on the gene promoter regions of 11 cell lines. Our results indicated that promoter methylomes could not only cluster cancer cell lines with respect to tissue origins but also differentiate cancer subtypes based on CpG island methylator phenotype (CIMP). Promoter-targeted LHC-BS as means for comprehensive screening and classifying cancer cells with promoter methylomes provided a powerful strategy for further complex clinical studies.
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