Journal
TALANTA
Volume 142, Issue -, Pages 145-149Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2015.04.058
Keywords
Quantum dot-beads; Immunochromatographic assay; Hepatitis B virus surface antigen (HBsAg); Quantitative determination
Categories
Funding
- National Key Technology Research and Development of the Ministry of Science and Technology of the People's Republic of China [2013BAD19B02]
- National Institutes of Health of United State [1R43AI092962]
- Training Plan for the Main Subject of Academic Leaders of Jiangxi Province [20142BCB22004]
- Training Plan for the Young Scientist (Jinggang Star) of Jiangxi Province [20142BCB23004]
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Hepatitis B virus infection is one of the major causes of hepatitis, liver cirrhosis and liver cancer. In this study, we used highly luminescent quantum dot-beads (QBs) as signal amplification probes in the sandwich immunochromatographic assay (ICA) for ultrasensitive and quantitative detection of hepatitis B virus surface antigen (HBsAg) in human serum. Various parameters that influenced the sensitivity and stability of the QB-based ICA (QB-ICA) sensor were investigated. Two linear independent regression equations for detection of serum HBsAg were expressed with Y=0.3361X-0.0059 (R-2=0.9983) for low HBsAg concentrations between 75 pg mL(-1) and 4.8 ng mL(-1), and Y=0.8404 X-2.9364 (R-2=0.9939) for high HBsAg concentrations in the range from 4.8 ng mL(-1) to 75 ng mL(-1). The detection limit of the proposed ICA sensor achieved was 75 pg mL(-1), which is much higher than that of the routinely-used gold nanoparticle based ICA. The intra- and inter-assays recovery rates for spiked serum samples at HBsAg concentrations of 75 pg mL(-1), 3.75 ng mL(-1) and 18.75 ng mL(-1) ranged from 90.14% to 97.6%, and coefficients of variation were all below 7%, indicating that the QB-ICA sensor has an acceptable accuracy for HBsAg detection. Additionally, the quantitative method developed showed no false positive results in an analysis of 49 real HBsAg-negative serum samples, and exhibited excellent agreement (R-2=0.9209) with a commercial chemiluminescence immunoassay kit in identifying 47 HBsAg-positive serum samples. In summary, due to its high fluorescence intensity, the sandwich QB-ICA sensor is a very promising point-of-care test for rapid, simple and ultrasensitive detection of HBsAg, as well as other disease-related protein biomarkers. (C) 2015 Elsevier B.V. All rights reserved.
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