Journal
TALANTA
Volume 144, Issue -, Pages 710-716Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2015.07.015
Keywords
Penicillins; Penicilloic acid; Surface enhanced Raman spectroscopy
Categories
Funding
- Egyptian Government [283]
- Austrian Research Funding Association (FFG) under the scope of the COMET program within the research network Process Analytical Chemistry (PAC) [825340]
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Elucidation and quantitative determination of some of commonly used penicillins (ampicillin, penicillin G and carbenicillin) in the presence of their main degradation product (penicilloic acid) were developed. Forced acidic and basic degradation processes were applied at different time intervals. The formed degradation products were elucidated and quantified using surface enhanced Raman spectroscopy (SERS). Silver nanoparticles (AgNPs) prepared by reduction of silver nitrate using hydroxylamine-HCl in alkaline medium were used as SERS substrate. The results obtained in SERS were confirmed by the application of LC/MS method. The concentration range was 100-600 ng/ml in case of the studied penicillins and 100700 ng/ml in case of penicilloic acid. An excellent correlation coefficient was found in case of ampicillin (r=0.9993) and in the case of penicilloic acid (r=0.9997). Validation procedures were carried out including precision, robustness and accuracy by comparing F- and t-values of both the proposed and reported methods. (C) 2015 Elsevier B.V. All rights reserved.
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