Journal
TALANTA
Volume 142, Issue -, Pages 176-182Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2015.04.037
Keywords
Fluorescent assay; Copper (II) determination; Copper-specific ssDNA; Sybr Green I
Categories
Funding
- National High Technology Research and Development Program (863 Program) of China [2012AA101405]
- Special Fund for Agro-scientific Research in the Public Interest of China [200903056]
- National Natural Science Foundation of China [31071860, 20977062]
- Municipal Natural Science Foundation of Shanghai, China [13ZR1421700]
- Morningstar Fund of Shanghai Jiaotong University [13X100010018]
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This paper reports a fluorescent turn-off assay for sensitive detection of Cu2+ in an aqueous solution by using a copper-specific ssDNA Cu100 and Sybr Green I. By monitoring the fluorescence changes arose from different interactions of Sybr Green I with Cu100 and Cu100/Cu2+ complex, the Cu2+ could be linearly detected from 5.57 to 250 ppb, with a detection limit of 5.57 ppb. The feasibility of this assay was demonstrated by detecting Cu2+ in certified reference materials and spiked water samples with satisfactory results. (C) 2015 Elsevier B.V. All rights reserved.
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