Journal
TALANTA
Volume 138, Issue -, Pages 225-230Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2015.02.056
Keywords
Fluorescence aptasensor; Label-free; DNA-sensitized luminescence of terbium (III); Cell detection
Categories
Funding
- National Natural Science Foundation of China [21375017, 21205015, 21105012]
- National Science Foundation for Distinguished Young Scholars of Fujian Province [2013J06003]
- Key Project of Fujian Science and Technology [2013Y0045]
- Program for Fujian University Outstanding Youth Scientific Research [JA11105, JA10295]
- Program for New Century Excellent Talents in University in Fujian Province [JA13130]
- Foundation of Fuzhou Science and Technology Bureau [2013-S-122-4]
- Medical Elite Cultivation Program of Fujian, P.R.C [2014-ZQN-ZD-26]
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Breast cancer is the most common type of malignant tumor in women. Recently, it has been shown that detection of breast cancer tumor cells outside the primitive tumor is an effective early diagnosis with great prognostic and clinical utility. For this purpose, we developed a signal-on fluorescence aptasensor for label-free, facile and sensitive detection of MCF-7 breast cancer cells. Due to target-aptamer specific recognition and single-stranded DNA-sensitized luminescence of terbium (III), the proposed aptasensor exhibits excellent sensitivity with detection limit as low as 70 cells mL(-1). Compared with common organic dyes and the emerging nano-technological probes, the combination of terbium (III) and single-stranded DNA signal probe (Tb3+-SP) serves as a more powerful bin-probe because of its stable optical property, good biocompatibility and free from complex synthesis. The feasibility investigations have illustrated the potential applicability of this aptasensor for selective and sensitive detection of MCF-7 breast cancer cells. Moreover, this proposed aptasensor can be also extended for the determination of other tumor cancers or bio-molecules by altering corresponding aptamers. Taken together, this easy-to-perform aptasensor may represent a promising way for early screening and detection of tumor cancers or other bio-molecules in clinical diagnosis. (C) 2015 Elsevier B.V. All rights reserved.
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