Journal
TALANTA
Volume 141, Issue -, Pages 97-102Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2015.03.021
Keywords
HL-60 cancer cells; CdS nanocrystals; Au nanoparticles; Energy transfer; Isothermal circular amplification; Electrochemiluminescence
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Funding
- National Natural Science Foundation of China [21135003, 21475058, 21121091]
- Science and Technology Support Program of Jiangsu Province, China [BE2013073]
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Here we have developed a sensitive cancer cell amplified detection method which combined Au NPs enhanced electrochemiluminescence (ECL) of CdS nanocrystals (NCs) film, with isothermal circular amplification reaction of polymerase. In DNA circular amplification detection system, hairpin DNA beacon/Au NPs composite modified CdS NCs film was used as an ECL emitter. Messenger DNA is hybridized with the aptamer modified on magnetic beads (MBs) to form MB-Au bioconjugates. In the presence of HL-60 cell, the aptamer would conjugate with the glycoprotein at cell surface and messenger DNA sequence would be released. The released messenger DNA sequence was then introduced into the cycle amplification system to trigger circular polymerizations. This assay allows us to determine the released messenger DNA equivalent to 10 cells and exhibits a significant specificity for HL-60 cells. (C) 2015 Elsevier B.V. All rights reserved.
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