Journal
NUTRITIONAL NEUROSCIENCE
Volume 17, Issue 5, Pages 214-221Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1179/1476830513Y.0000000083
Keywords
Multiple sclerosis; Inflammation; Intracellular Ca2+ concentration; Oxidative stress; Neutrophils; TRP Channels
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Funding
- Scientific Research Unit of Suleyman Demirel University [2529-M-10]
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Objectives: Hypericum perfortarum (HP, St John's wort) is a modulator of Ca2+ entry in neutrophils and it may modulate intracellular free Ca2+ ([Ca2+](i)) entry in leukocytes of patients with multiple sclerosis (MS). We investigated effects of HP on oxidative stress, apoptosis, and [Ca2+](i) concentrations in serum and leukocytes of patients with MS. Methods: Neutrophils of nine newly diagnosed MS patients and nine healthy subjects within four subgroups were used in the study. The first group was a control; the second group was patients with MS. The neutrophils from patient group were incubated non-specific TRPM2 channel blocker (2-APB), voltage-gated calcium channel blockers, verapamil and diltiazem (V + D) with HP before N-formyl-L-methionyl-L-leucyl-L-phenylalanine stimulation, respectively. Results: Neutrophil and serum lipid peroxidation, neutrophil apoptosis and [Ca2+](i) levels in patients with MS were higher than in control although their levels were decreased by HP, 2-APB, and V + D incubations. The modulator role of V + D in MS and MS + HP groups was higher than in the 2-APB group. Neutrophilic glutathione peroxidase (GSH-Px) and serum vitamin A and E concentrations were lower in the MS group than in control. However, the neutrophil GSH-Px activity was increased by HP incubation. The neutrophil reduced glutathione, serum vitamin C and beta-carotene concentrations did not change in control and patients. Discussion: We observed that HP-induced protective effects on oxidative stress and [Ca2+](i) concentrations by modulating transient receptor potential and voltage gated calcium channel in the patients with MS. Thus, it may provide useful treatment of neutrophil activity in the patients.
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