Journal
NUTRITIONAL NEUROSCIENCE
Volume 15, Issue 5, Pages 34-41Publisher
MANEY PUBLISHING
DOI: 10.1179/1476830512Y.0000000011
Keywords
Alpha-mangostin; Cerebellar granule neurons; Curcumin; Heme oxygenase-1; Reactive oxygen species
Categories
Funding
- CONACyT (Consejo Nacional de Ciencia y Tecnologia, Mexico) [129838]
- PAPIIT (DGAPA-UNAM) [IN201910]
Ask authors/readers for more resources
Curcumin is a phenolic yellow curry pigment with anti-inflammatory and antioxidant activities and alpha-mangostin is a xanthone isolated from mangosteen fruit with antioxidant properties. Iodoacetate (IAA) is an inhibitor of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase that induces a model of metabolic inhibition in neurons where reactive oxygen species (ROS) production is a significant mechanism. Furthermore, it has been shown that the induction of heme oxygenase-1 (HO-1) protects against IAA-induced neuronal death. Objectives: To study the effects of alpha-mangostin and curcumin against the IAA-induced cell death and on HO-1 expression in primary cultures of cerebellar granule neurons (CGNs). Methods: CGNs were treated with curcumin or alpha-mangostin before the addition of IAA. Cell viability and ROS production were measured 24 and 4 hours after IAA addition, respectively. HO-1 expression was measured by western blot. Results: Both alpha-mangostin and curcumin pretreatment ameliorated the neuronal death induced by IAA in a concentration-dependent way, which was associated with an amelioration of IAA-induced ROS formation. In addition, it was found that alpha-mangostin and curcumin induced HO-1 expression. Discussion: Treatment with alpha-mangostin and curcumin provided a neuroprotective effect against IAA in primary cultures of CGNs, an effect associated with an amelioration of the IAA-induced ROS production. HO-1 induced by these antioxidants may also be involved in the neuroprotective effect. Future work will be required to determine whether alpha-mangostin may cross the blood-brain barrier and achieve enough bioavailability to elicit a protective response in the brain being an effective nutraceutical compound for preventive therapy of neurodegenerative diseases.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available