Journal
TALANTA
Volume 139, Issue -, Pages 226-232Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2015.02.036
Keywords
Aptamer; Fluorescence; Gold nanoparticles; Kanamycin A
Categories
Funding
- National Natural Science Foundation of China [21205108]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars (State Education Ministry of China)
- Technology Foundation for Selected Overseas Chinese Scholars (Ministry of Personnel of China)
- Special Fund for Agro-scientific Research in the Public Interest [201203094]
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In this study, a simple and sensitive aptamer-based fluorescence method for the detection of Kanamycin A by using gold nanoparticles (AuNPs) has been developed. In this assay, AuNPs were utilized as DNA nanocarrier as well as efficient fluorescence quencher. In the absence of Kanamycin A, dye-labeled aptamer could be adsorbed onto the surface of AuNPs and the fluorescence signal was quenched. In the presence of Kanamycin A, the specific binding between dye-labeled aptamer and its target induced the formation of rigid structure, which led to dye-labeled aptamer releasing from the surface of AuNPs and the fluorescence intensity was recovered consequently. Under optimum conditions, calibration modeling showed that the analytical linear range covered from 0.8 nM to 350 nM and the detection limit of 0.3 nM was realized successfully. This proposed bio-assay also showed high selectivity over other antibiotics. Meanwhile, this strategy was further used to determine the concentrations of Kanamycin A in milk sample with satisfying results. (C) 2015 Elsevier B.V. All rights reserved.
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