Journal
TALANTA
Volume 132, Issue -, Pages 72-76Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2014.08.064
Keywords
Copper; Click chemistry; Fluorescence; Molecular beacon
Categories
Funding
- National Natural Science Foundation of China [21275139]
- National Basic Research Program of China (973 Program) [2011CB911002]
- State Key Laboratory of Supramolecular Structure and Materials [SKLSSM201415]
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In this work, a fluorescence turn-on method for copper(II) detection is reported. A molecular beacon (MB) was designed as a template. Cu2+ was reduced to Cu+ in the presence of a reductant (ascorbic acid). Two short single-stranded oligonucleotides one was labeled with a 5'-alkyne and the other with 3'-azide group, proceeded a template-dependent chemical ligation through the Cu(I)-catalyzed azide-alkyne cycloaddition. The newly generated click-ligated long chain oligonucleotide, which was complementary to the MB, opened the MB hairpin structure and resulted in a turn on fluorescence. The increase in fluorescence intensity is directly proportional to the amount of Cu2+ added to the assay solution. The present assay is quite sensitive and allows the detection of 2 nM Cu2+. The described assay also exhibits high selectivity over other metal ions. (C) 2014 Elsevier B.V. All rights reserved.
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