Journal
TALANTA
Volume 143, Issue -, Pages 402-413Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2015.05.012
Keywords
Amlodipine; Sensing agent; Cyclodextrin; Surfactants; Nanoparticles
Categories
Ask authors/readers for more resources
Four native fluorescence methods were suggested for simultaneous determination of amlodipine (AML) and valsartan (VAL). These methods were based on excitation of both drugs at lambda(ex) 300 nm, in one step, to give maximum emission at lambda(em) 378 and 496 nm for AML and VAL, respectively. The first method, single lambda(ex). method, was used without any additions. The sensitivity of this method was further increased by the addition of hydroxy propylmethyl cellulose (HPMC) surfactant, beta-cyclodextrin, or ferric oxide magnetite nanoparticles, in the other three methods. Different types of surfactants, and different concentration levels of both beta-cyclodextrin and ferric oxide nanoparticles, were scanned to determine the optimum conditions for enhancing the sensitivity. Some factors affecting the fluorescence intensity of both cited drugs, like the type and volume of the added solvent (to be used as a sensing agent), and pH of measurement were studied and optimized. The proposed methods could be used in determination of AML and VAL in bulk powder, their laboratory prepared mixtures and pharmaceutical formulations. The obtained results were statistically compared to each other and to that of some reported methods. The specificity of the developed methods was investigated, and the methods were validated according to ICH guidelines. (C) 2015 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available