Journal
SYSTEMATIC AND APPLIED MICROBIOLOGY
Volume 38, Issue 6, Pages 373-378Publisher
ELSEVIER GMBH
DOI: 10.1016/j.syapm.2015.06.001
Keywords
Agrobacterium; Crown gall; 16S rDNA; MLSA; ANI; Taxonomy
Categories
Funding
- Ministry of Education, Science and Technological Development, Republic of Serbia [III46008]
- EU [316004]
- National Science Centre, Poland [DEC-2013/08/M/NZ9/00138]
Ask authors/readers for more resources
Two plant-tumorigenic strains KFB 330(T) and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacterium, with A nepotum as their closest relative. Multilocus sequence analysis (MLSA) based on the partial sequences of atpD,glnA, gyrB, recA and rpoB housekeeping genes suggested that these three strains represent a new Agrobacterium species, that clustered with type strains of A. nepotum, A. radiobacter, A fabrum and A. pusense. This was further supported by average nucleotide identity values (<92%) between the whole genome sequences of strain KFB 3301 and related Agrobacterium species. The major cellular fatty acids of the novel strains were 18:1 w7c (72.8-77.87%) and 16:0 (6.82-8.58%). Phenotypic features allowed their differentiation from closely related species. Polyphasic characterization showed that the three strains represent a novel species of the genus Agrobacterium, for which the name Agrobacterium arsenijevicii sp. nov. is proposed. The type strain ofA. arsenijevicii is KFB 330T (=CFBP 8308(T) = LMG 28674(T)). (C) 2015 Elsevier GmbH. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available