Journal
SYSTEMATIC AND APPLIED MICROBIOLOGY
Volume 38, Issue 1, Pages 48-55Publisher
ELSEVIER GMBH
DOI: 10.1016/j.syapm.2014.09.003
Keywords
Biodiversity; Bacterial community; Biodeterioration; Molecular methods; 16S rRNA clone libraries; Unculturable bacteria
Categories
Funding
- Auschwitz-Birkenau Foundation
- Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Poland
Ask authors/readers for more resources
The aim of this work was to analyze the bacterial communities in four samples of historical materials (plaster, brick, and wood) derived from buildings located in the former Auschwitz II-Birkenau concentration and extermination camp in Brzezinka, Poland. For this purpose a molecular strategy based on the construction of 16S rRNA clone libraries was used. In total. 138 partial 16S rRNA gene sequences (-600 bp) were obtained and compared. The clones belonged to phyla Proteobacteria (classes: Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria), Actinobacteria, Firmicutes, and Bacteroidetes. The plaster samples predominantly contained clones closely related to Actinobacteria and Alphaproteobacteria, brick samples contained Gammaproteobacteria, while wood samples had Actinobacteria clones. Interestingly, the historic plaster and brick samples contained the following bacteria with known and described biodeterioration potential: chemoorganotrophic Streptomyces sp. and Pseudonocardia sp.. halotolerant or halophilic Rubrobacter sp., Salinisphaera sp. and Halomonas sp. Principal component analysis (PCA) showed that amongst the bacterial species detected and identified none occurred on all the tested historical materials. The 165 rRNA clone library construction method was successfully used for the detection and diversity determination of bacterial communities inhabiting brick barracks located in the former Auschwitz II-Birkenau concentration and extermination camp in Brzezinka. (C) 2014 Elsevier GmbH. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available