Journal
NUTRITION
Volume 25, Issue 7-8, Pages 782-789Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.nut.2008.12.004
Keywords
Trans-fatty acids; Polyunsaturated fatty acids; Adipokines; Peroxisome proliferator-activated receptor-gamma; Adipose tissue; Rats; Glucose; Lipid homeostasis
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Funding
- Universidad San Pablo-CEU [19/03]
- Ministerio de Educacion y Ciencia of Spain [SAF2004-05998]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/CNPq [471602/2004-3]
- Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro-FAPERJ [E-26/170.605/2007]
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Objective: Our previous study indicated that partially hydrogenated fat (PHF) diets, rich in trans-isomers, alter plasma lipids and increase the lipogenesis rate on adipose tissue in rats at a young age. In the present study we investigated the effects of dietary PHF on the expression of genes associated with glucose and lipid metabolism in rat adipose tissue. Methods: Female Wistar rats were fed normolipidic diets containing PHF (rich in trans-fatty acids and poor in polyunsaturated fatty acids [PUFAs]), soy oil (rich in omega-6 PUFAs), and fish oil (rich in omega-3 PUFAs) during gestation and lactation; young male pups were fed the same diets from weaning until 120 d of life. The mRNA expression of peroxisome proliferator-activated receptor-gamma, tumor necrosis factor-alpha, resistin, adiponectin, and leptin were analyzed in retroperitoneal adipose tissue (RET) using real time polymerase chain reaction. Results: The PHF group showed the highest triacyl glycerol, glucose, and insulin levels and the lowest plasma adiponectin level. The RET of PHF incorporated trans-fatty acids, whereas fish oil and soy oil groups had increased omega-3 and omega-6 PUFAs, respectively. In the RET the PHF group had the highest resistin and tumor necrosis factor-alpha levels and the lowest adiponectin and peroxisome proliferator-activated receptor-gamma gene expressions, whereas the fish oil group had the highest peroxisome proliferator-activated receptor-gamma and the lowest leptin gene expression. Conclusion: Prolonged intake of PHF has a negative effect on the expression of genes in RET when compared with diets with omega-6 and omega-3 PUFAs. These changes may be an effect of the smaller proportions of PUFAs in this fat, instead of being only caused by trans-fatty acids. Published by Elsevier Inc.
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