Journal
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
Volume 27, Issue 9, Pages 1084-1096Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15257770802271813
Keywords
DNA triplex; terpyridine; metal ion; UV melting; amidite reagent
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The flexible polypyridine ligand, 2,2':6',2 ''-terpyridine (terpy), was built into the backbone of oligonucleotides to form DNA conjugates. The terpy unit functioned as a good loop when the conjugates formed the bimolecular triplexes with complementary oligopurine. The triplex structure was destabilized by the specific interaction with divalent transition metal ions (Cu2+, Zn2+, and Fe2+), in particular Cu2+ ions. This ion destabilized one of the triplexes by 4.2 kcalmol(-1) or made the triplex formation constant less than 1/10(3) at 298 K. This result is attributed to the substantial turbulence of the terminal structure of the triplexes.
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