Journal
NUCLEIC ACIDS RESEARCH
Volume 42, Issue 22, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gku945
Keywords
-
Categories
Funding
- National Institutes of Health [ES017010, ES002109]
- National Science Foundation [CHE-1308839]
- National Research Foundation of Singapore under its Singapore-MIT Alliance for Research and Technology
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1308839] Funding Source: National Science Foundation
Ask authors/readers for more resources
Emerging evidence points to roles for tRNA modifications and tRNA abundance in cellular stress responses. While isolated instances of stress-induced tRNA degradation have been reported, we sought to assess the effects of stress on tRNA levels at a systems level. To this end, we developed a next-generation sequencing method that exploits the paucity of ribonucleoside modifications at the 3 '-end of tRNAs to quantify changes in all cellular tRNA molecules. Application of this tRNA-seq method to Saccharomyces cerevisiae identified all 76 expressed unique tRNA species out of 295 coded in the yeast genome, including all isoacceptor variants, with highly precise relative (fold-change) quantification of tRNAs. In studies of stress-induced changes in tRNA levels, we found that oxidation (H2O2) and alkylation (methylmethane sulfonate, MMS) stresses induced nearly identical patterns of up-and down-regulation for 58 tRNAs. However, 18 tRNAs showed opposing changes for the stresses, which parallels our observation of signature reprogramming of tRNA modifications caused by H2O2 and MMS. Further, stress-induced degradation was limited to only a small proportion of a few tRNA species. With tRNA-seq applicable to any organism, these results suggest that translational control of stress response involves a contribution from tRNA abundance.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available