Journal
NUCLEIC ACIDS RESEARCH
Volume 42, Issue 6, Pages 3894-3907Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkt1361
Keywords
-
Categories
Funding
- Swiss National Science Foundation [CRSI33 125073]
- European Molecular Biology Organization [AST140-2013]
- European Molecular Biology Organization [Fundacion La Caixa]
- Spanish Ministry of Economy and Competitiveness [BIO2012-39980]
- European Research Council [DHISP250128]
- University of Zurich
- Swiss National Science Foundation (SNF) [CRSI33_125073] Funding Source: Swiss National Science Foundation (SNF)
Ask authors/readers for more resources
Conditional mutagenesis using Cre recombinase expressed from tissue specific promoters facilitates analyses of gene function and cell lineage tracing. Here, we describe two novel dual-promoter-driven conditional mutagenesis systems designed for greater accuracy and optimal efficiency of recombination. Co-Driver employs a recombinase cascade of Dre and Dre-respondent Cre, which processes loxP-flanked alleles only when both recombinases are expressed in a predetermined temporal sequence. This unique property makes Co-Driver ideal for sequential lineage tracing studies aimed at unraveling the relationships between cellular precursors and mature cell types. Co-InCre was designed for highly efficient intersectional conditional transgenesis. It relies on highly active trans-splicing inteins and promoters with simultaneous transcriptional activity to reconstitute Cre recombinase from two inactive precursor fragments. By generating native Cre, Co-InCre attains recombination rates that exceed all other binary SSR systems evaluated in this study. Both Co-Driver and Co-InCre significantly extend the utility of existing Cre-responsive alleles.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available