Journal
NUCLEIC ACIDS RESEARCH
Volume 42, Issue 4, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkt1160
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Funding
- Russian Foundation for Basic Research [11-04-01018-a, 11-04-01314-a, 12-04-33026-mol-a-ved, 13-04-00836-a, 14-04-01061-a, 13-04-40211-N, 12-04-31363-mol-a]
- Ministry of Education and Science of Russian Federation
- Moscow University Development Program PNR [5.13]
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Chemical landscape of natural RNA species is decorated with the large number of modified nucleosides. Some of those could easily be detected by reverse transcription, while others permit only high-performance liquid chromatography or mass-spectrometry detection. Presence of m(6)A nucleoside at a particular position of long RNA molecule is challenging to observe. Here we report an easy and high-throughput method for detection of m(6)A nucleosides in RNA based on high-resolution melting analysis. The method relies on the previous knowledge of the modified nucleoside position at a particular place of RNA and allows rapid screening for conditions or genes necessary for formation of that modification.
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