Journal
NUCLEIC ACIDS RESEARCH
Volume 42, Issue 4, Pages 2512-2524Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkt1184
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Funding
- Clermont University
- Centre National pour la Recherche Scientifique (CNRS)
- Institut National pour la Sante et la Recherche Medicale (INSERM)
- Ministry of Education
- Region Auvergne [Nouveau chercheur]
- European Union
- Agence pour la Recherche contre le Cancer (ARC)
- Ligue regionale contre le Cancer
- INSERM U1103
- CNRS 6293
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During Drosophila oogenesis, transposable element (TE) repression involves the Piwi-interacting RNA (piRNA) pathway which ensures genome integrity for the next generation. We developed a transgenic model to study repression of the Idefix retrotransposon in the germline. Using a candidate gene KD-approach, we identified differences in the spatio-temporal requirements of the piRNA pathway components for piRNA-mediated silencing. Some of them (Aub, Vasa, Spn-E) are necessary in very early stages of oogenesis within the germarium and appear to be less important for efficient TE silencing thereafter. Others (Piwi, Ago3, Mael) are required at all stages of oogenesis. Moreover, during early oogenesis, in the dividing cysts within the germarium, Idefix anti-sense transgenes escape host control, and this is associated with very low piwi expression. Silencing of P-element-based transgenes is also strongly weakened in these cysts. This region, termed the 'Piwiless pocket' or Pilp, may ensure that new TE insertions occur and are transmitted to the next generation, thereby contributing to genome dynamics. In contrast, piRNA-mediated silencing is strong in germline stem cells in which TE mobilization is tightly repressed ensuring the continued production of viable germline cysts.
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